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Title: Development of Photobiomodulation (PBM) approaches for the management of oral diseases
Author: Serrage, Hannah
ISNI:       0000 0004 9359 0731
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2019
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Photobiomodulation (PBM) is a non-invasive phototherapy that has shown promise in the treatment of the chronic oral inflammatory disease, periodontitis. This project aimed to provide evidence of a well characterised system for the evaluation of the effects of PBM on the most abundant cell type in the oral periodontium: primary human gingival fibroblasts (pHGFs). Two bespoke high throughput light arrays were extensively characterised (2nd generation array: 400-830 nm, 24mW/cm2 and LUMOS® array: 405-850 nm, 24mW/cm2) using techniques including beam profilometry and UV-Vis spectrometry. The biological effects of PBM on pHGFs (either single isolates/pooled from 3 individuals) were evaluated following application of media or periodontal disease relevant stimuli. The effects of PBM were biomodulatory, where application of blue light (400-450 nm, 24mW/cm2, 5.76J/cm2) to ‘healthy’ pHGFs induced increases in markers for inflammation (IL-8, ROS) but application of PBM to ‘disease’ pHGFs (bacterial stimulus applied) caused decreases in markers for inflammation. PBM also modulated mitochondrial activity, where pHGFs with a higher mitochondrial content exhibited greater maximal and basal respiratory rates. These data indicate blue light could be an effective modality in the modulation of inflammation in periodontal disease. Results also provide novel evidence that mitochondrial content could be a prerequisite in ensuring a response to PBM in vivo.
Supervisor: Not available Sponsor: EPSRC
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Q Science (General) ; RK Dentistry