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Title: Functions of ZNF516 in embryonic development, health, and disease
Author: Przewrocka, Joanna Paula
ISNI:       0000 0004 9359 891X
Awarding Body: UCL (University College London)
Current Institution: University College London (University of London)
Date of Award: 2020
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Zinc finger protein 516, or ZNF516, is a largely understudied protein. Germline homozygous loss of the murine ortholog, ZFP516, has been previously reported to lead to embryonic lethality in mice. However, the cause of this lethality has not been investigated. With the aid of newly developed Zfp516 knock out mouse line in our laboratory I characterised the phenotypes of Zfp516 knock out animals and concluded that severe and 100% penetrant congenital heart defects were the most likely cause of embryonic lethality in Zfp516-/-. I reported that heart defects were not due to selective Zfp516 deletion in cardiomyocytes, and that only some defects were recapitulated when Zfp516 was deleted in Isl1-expressing cells. I further described spatiotemporal expression of Zfp516 during development and showed that Zfp516 ablation at E9.5 leads to upregulation of genes involved in neuronal migration, growth and synapsogenesis. Moreover, I found genetic synergy between Zfp516 and Sox10 during development. Next, I characterized phenotypes associated with deletions of and polymorphisms in human ZNF516 and adult heterozygous Zfp516 knock out mice. I discovered that manifestation of phenotypes in Zfp516+/- mice was largely gender dependent, and that our mouse model phenocopied some of the phenotypes seen in humans. Lastly, I reported that targeting ZNF516 in HCT116 with CRIPSR/Cas9 can result in chromosome arm truncations. I therefore suggested a screening method for future studies that could preselect clones with correct genotype and without arm truncations before further cell expansion for subsequent downstream experiments.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available