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Title: T lymphocyte responses to glycoprotein B of herpes simplex virus type 1
Author: O'Donnell, Catherine Agnes
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 1990
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Glycoprotein B (gB) of herpes simplex virus type 1 (HSV-1) is expressed on the virus envelope and on the surface of infected cells, It is an important viral protein, both functionally and as a targst. nor the immune response of the infected host. Although gB of HSV-1 (gB-1) does not induce viral neutralising antibody, subcutaneous pruning with immunopurified gB-1 can protect mice against a lethal infection with IISV-1. This protective effect could be adoptively transferred by specific T helper cells. This project further investigated the role of T cells specific for HSV-1 and gB-1 in the response to the virus. This was carried out in three areas 1. A comparison of the T cell response to, and induced by, gB-1 and its unglycosylated precursor form, pgB-1, to determine if glycosylation of gB had any effect on the T cell response. T cell proliferation assays showed that HSV primed cells could proliferate equally well to gB or pgB in vitro. Priming with pgB in vivo induced virus and glycoprotein specific T cells as effectively as gB, and could protect mice against a lethal challenge with HSV-1. Therefore, glycosylation is not important in the recognition of gB by T helper ceiis, either in vitro or in vivo. 2. The production of lymphokines (IL-l, IL-2, IL-3 and IFN-γ ) in vivo, post infection, in mice primed with whole virus, gB-1 or pgB-1 was examined. Again, pgB was as effective as gB in inducing lymphokine production. Priming appeared to induce earlier, elevated lymphokine levels compared to that seen in the control mice. However, control mice which survived beyond 10 days post infection developed lymphokine levels comparable t:o -chose detected in the primed animals. Thus, the early production of lymphokines in the primed animals may contribute to their protection against infection. 3. A panel of T cell clones were developed which recognized HSV-1 and, in some cases, gB-1. These were CD4+ and I-Ad restricted. In response to antigen restimulation in vitro, all the clones produced , IL-3 and IFN-γ. Therefore, the clones could be tentatively classed as Th cells.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available