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Title: Characterization of nuclear segregation of RNA as a potential novel marker and regulation mechanism of quiescence in neural stem cell
Author: Coum, Antoine
ISNI:       0000 0004 9357 2701
Awarding Body: King's College London
Current Institution: King's College London (University of London)
Date of Award: 2019
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Characterization of quiescent stem cells is a crucial step in manipulating quiescence in tissue regeneration and repair, and cancer treatment. Through the characterization of a Drosophila melanogaster mutant, I uncovered a potential novel regulation mechanism of neural stem cell quiescence. I observed that downregulation of Nucleoporins and karyopherins induces quiescence in Drosophila melanogaster neural stem cells. Nucleocytoplasmic transport components appeared altered in vivo in Drosophila neural stem cell and in vitro in a cell culture model of mouse adult hippocampal neural stem cell. I thus hypothesized that transport of particular cargo should be altered in quiescent neural stem cells, and demonstrated that polyadenylated RNA is segregated to the nucleus in quiescent neural stem cells in both models. I also started to characterize targets linked to this segregation mechanism, and showed that nuclear segregation of polyadenylated RNA occurred in mouse muscle satellite cells. Although much characterization of this novel mechanism in stem cell quiescence still has to be done, I offer a view of a potential novel regulator as well as a potential novel marker of quiescence.
Supervisor: Sousa-Nunes, Rita Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available