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Title: On the microtubule-based transport of Marek's Disease Virus
Author: Tut, Gokhan
ISNI:       0000 0004 9357 7916
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2018
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Viruses hijack the host machinery for their proliferation and spread. This includes the host cytoskeleton. Alphaherpesvirus viral capsids are assembled in the nucleus of an infected cell and then require to be trafficked to various host compartments for tegumentation, secondary envelopment and egress. Microtubules are important for long-distance transport of viral particles inside host cells. Marek's Disease Virus (MDV) is an Alphaherpesvirus and is the causative agent of a lymphoproliferative disease called Marek's Disease (MD) in poultry. Here, we demonstrate that both microtubules and actin are required for cell-to-cell spread of MDV in culture. We show that MDV infection induces cell shape changes and these changes depend on dynamic microtubules. Using live cell super-resolution microscopy, we show that the majority of MDV viral particles are associated with microtubules and that MDV particles are transported along microtubules. Cytoplasmic dynein and KIF13B molecular motors have been identified to contribute to the directional transport of MDV viral particles along microtubules. Thus, the findings from this study identify new components of the host machinery required for MDV biology and open new avenues of research for the MDV field.
Supervisor: Not available Sponsor: Medical Research Council
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QH Natural history ; QR Microbiology ; SF Animal culture