Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.809402
Title: Exploring the effects of ageing on the host-microbiota axis skin repair
Author: Bornikoel, Anna
Awarding Body: University of Manchester
Current Institution: University of Manchester
Date of Award: 2019
Availability of Full Text:
Access from EThOS:
Access from Institution:
Abstract:
The prevalence of perturbed wound healing, characterised by dysregulated inflammation and often accompanied by bacterial infections, is increased with progressing age. Age-related changes in the skin microbiota or the response to bacterial products may contribute to the dysregulated inflammatory and perturbed wound healing response. This was explored by examining the expression and function of pattern recognition receptors (PRRs), which initiate inflammation in response to bacterial product exposure, in vitro and in vivo as well as their potential impact on the wound healing response with increased age. In vitro PRR stimulation of neutrophils and macrophages from wounded mice demonstrated age-related differences in PRR and downstream cytokine expression (TNF, IL-Î2, IL-6 and IL-12) with increased age. Although 5-7 week and 18 month old mice showed similar responses to PRR ligand stimulation, 22-24 month old mice showed an impaired response. Examination of PRR expression and function with increasing age further highlighted the importance of considering the temporal aspect of the response to PRR ligand treatment, as changes in PRR expression and function with increased age differed considerably in response to 3h compared to 24h treatment. Interestingly, TLR1, TLR2 and TLR4 mRNA expression was significantly increased in untreated wounds of aged (22-24 month old) compared to young (5-7 weeks) mice, while the wound healing response to PRR ligand treatment in aged was impaired. Thus, the results presented here demonstrate age-related changes in response to PRR ligand exposure, which may contribute to the dysregulated inflammatory response associated with impaired wound healing in the aged, as well as potentially delaying other aspects of wound healing.
Supervisor: Mcbain, Andrew ; Mace, Kimberly Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.809402  DOI: Not available
Keywords: PRR and downstream cytokine expression ; PRR signalling ; Age-related changes ; Skin microbiota ; Wound healing ; Ageing
Share: