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Title: Role of oestradiol on function of lymphocytes from normal donors and patients with common variable immunodeficiency
Author: Evangelatou, Marianthi
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 1995
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The present work has compared the functional effects of oestradiol on the function of lymphocytes from normal individuals and patients with the primary immunodeficiency disease, common variable immunodeficiency. In order to obtain a comprehensive assessment about the role of oestradiol on normal lymphoid cell populations, lymphocytes were separated from human peripheral blood, spleen and tonsils. The responses of lymphocytes to oestradiol were shown to be dependent on the tissue source with most variability observed with blood. This variability may be linked to the different serum hormonal levels of male and female donors and the different hormonal status between female donors. Principally, significant stimulatory effects were found on immunoglobulin production but under some conditions oestradiol was also inhibitory. The action of oestradiol on immunoglobulin production is shown to be T cell dependent, to require intact T cells and to be modulated by different ratios of CD4+ and CD8+ T cells. In addition, direct T cell responses were enhanced by oestradiol, i.e. mitogen-driven DNA synthesis. Lymphocytes from patients with common variable immunodeficiency were shown to be defective in their responses to oestradiol in this T cell-B cell system. The most probable mechanism for the action of oestradiol on immunoglobulin production by B cells is by modulating genes in T cells and/or accessory cells which are involved in the cytokine network important for T cell-B cell interaction. Data from the present project suggest that oestradiol modulates lymphocyte activation antigens, cytokine genes (at the mRNA level) and is also able to down-regulate its own receptor. However, further studies will be needed to extend the current knowledge of the complex mechanism of oestradiol action on the immune system.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available