Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.807137
Title: Proton and carbon-13 NMR lipid profiles of cells, tissues and body fluids
Author: Choi, Gregory Tak-Yu
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 1994
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Abstract:
This thesis describes the development of a novel, comprehensive and rapid biological lipid analysis via high-field NMR. Based upon the information obtained from various 2-dimensional (2D) NMR experiments, and by comparing NMR spectra of biological lipid extracts to standard lipid spectra, plus cross-references with existing data when available, the major lipids and fatty acids have been successfully identified and quantified. Significantly, information obtained from NMR experiments proved to be in agreement with previous published data, which was obtained using well-established but time-consuming conventional chromatographic techniques. The NMR lipid assay was then applied to the analysis of various biological tissues, cells and body fluids including rat liver, brain, heart, kidney, mast cell, human erythrocyte and plasma lipid extract. Assignments were mostly achieved using 1H- and 13C-NMR spectroscopy, although experiments involved other nuclei were also performed when necessary (e.g. 31P). Applications to the detection of lipid and phospholipid changes in carbon tetrachloride treated animals were carried out.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.807137  DOI: Not available
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