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Title: Protein transport and the reassembly of the Golgi apparatus during telophase in HeLa cells
Author: Souter, William Ewen Logan
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 1993
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At the onset of M phase in animal cells, the Golgi apparatus undergoes disassembly. The pathway of disassembly involves the generation of several hundred discrete Golgi stacks which break down further to yield vesicles. These vesicles are dispersed throughout the mitotic cytoplasm which subsequently provide the building blocks for the reassembly of two daughter organelles during telophase. Mitosis is also characterised by the arrest of a number of membrane-mediated transport processes including transfer of newly synthesised protein from the endoplasmic reticulum to the Golgi apparatus. The kinetic relationship between the resumption of this protein transport with the reassembly of the Golgi stack during telophase has been investigated in HeLa cells. Prometaphase- arrested cells were pulse-labelled with 35S-methionine and chased in the absence of nocodazole to allow passage through mitosis and into G1. Resumption of transport of histocompatibility antigen (HLA) molecules to the medial and trans Golgi cisternae was measured by monitoring the resistance to endoglycosidase H and the acquisition of sialic acid residues respectively. Transport to the plasma membrane was measured using neuraminidase to remove sialic acid residues on surface HLA molecules. The half-time for transport to each of these compartments was about 70 min longer in cells progressing out of mitosis than in G1 cells. The half-time for reassembly of the Golgi stack, quantified using stereological procedures, was also 70 min, suggesting that both transport and reassembly are triggered at the same time. However, since reassembly is a more rapid process than the delivery of protein from the ER to the Golgi apparatus, the Golgi stack has reassembled by the time newly synthesised protein reaches it.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available