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Title: Applied data science methods in epitranscriptomic bioinformatics
Author: Wei, Zhen
Awarding Body: University of Liverpool
Current Institution: University of Liverpool
Date of Award: 2020
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Chemical modifications on messenger RNA have been recently revealed by biological researchers to function as an essential layer of gene expression regulation. Molecular biologists from different laboratories have conducted more than 200 sets of high throughput sequencing experiments trying to capture the types and locations of messenger RNA modifications across multiple cell types and species. However, until this date, the field still lacks a bioinformatics pipeline to quantify and analyze the epitranscriptomic HTS data generated from different laboratories consistently. The thesis aims to provide an overview of questions and challenges arisen in the field of mRNA modification computational analysis. Subsequently, we will present a set of practical computational strategies for data explorations, genomic data mining, modification level quantifications, and technical artifact corrections from a data science perspective. The first chapter of the thesis provides an in-depth data exploration and visualization of m5C mRNA modification from bisulfite sequencing data. In the second chapter, we document the database construction and data consistency exploration for the transcriptomic targets of the mRNA modification related protein regulators. Besides, the second chapter presents a methodological framework for the computational representation of the domain knowledge related to the transcriptomic topology of epitranscriptomic modification. The final section of the thesis discusses the dominant technical biases existed in MeRIP-Seq, the most widely applied type of HTS data in epitranscriptomics, and it follows with a practical computational pipeline to overcome the technical error.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral