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Title: Molecular recognition of anionic species in aqueous environments
Author: Bole, Amanda
ISNI:       0000 0004 8508 1742
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2019
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This thesis focuses on sensing anions in complex medium and selective extraction of anions from complex media. Chapter 2 examined the interaction between polymerisable squaramides and phosphate ions. The squaramides displayed a strong colour change in the presence of phosphate in organic solvents, this colour change was successfully transferred to subsequent synthesised polymers. However, in aqueous media no colour change was observed. Cationic polymers were investigated and found to show no colour change but strong binding. Chapter 3 then focused on the interactions of both cationic and polymerisable squaramides working together to bind and sense phosphate. The polymer composition was optimised and the resultant polymer produced a colour change in the presence of phosphate in water. Chapter 4 then looks at the problem of biosample analysis on analytical instruments. In this chapter I developed a number of polymer formats for removal of phospholipids, these included grafting polymers on glass surfaces and on polymer beads. The most practical and applicable was the simplest, C-18 silane attached to a glass surface, this material allowed for phospholipid removal from the sample and washing the surface with methanol allowed for complete removal of the phospholipids regenerating the surface for use again. Chapter 5 then took the methods of surface functionalisation and applied it towards the selective removal of Circulating Tumour Cells (CTCs) from body fluids. This chapter looked at incorporating size and shape selectivity, through surface macro-imprinting with polymer beads that acted as CTC mimics. Molecular recognition by grafting polymers designed to bind with the anionic cell surface from the dimpled surfaces found that different polymers bound different cell lines leading to an exciting possibility of introducing selectivity for CTCs from different cancer origins.
Supervisor: Manesiotis, Panagiotis ; Kennedy, Richard Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available