Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799532
Title: The interactions between bacteria, platelets bags and platelets
Author: Wilson-Nieuwenhuis, Joels
ISNI:       0000 0004 8505 2220
Awarding Body: Manchester Metropolitan University
Current Institution: Manchester Metropolitan University
Date of Award: 2018
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Abstract:
Bacterial contamination of blood products is a major risk in transfusion medicine, with bacterial attachment and biofilm formation a potential source for false negative results of bacterial platelet contamination. Understanding platelet bag (PB) surface properties could improve understanding of how bacteria adhere to these surfaces. PB surfaces were analysed using scanning electron microscopy, optical surface profiling, Fourier transform infrared spectroscopy, RAMAN spectroscopy, goniometry, energy dispersive X-ray and a tensiometer. Initial findings demonstrated that the PBs had two distinct surfaces, a rough diamond patterned surface and a smoother surface. Two surface altering methods were used; PBs surfaces were flattened to remove surface features, which can increase bacterial attachment, and treated with low temperature atmospheric pressure plasma, with flattening demonstrating surface feature changes and plasma treatment being unsuitable. Staphylococcus epidermidis and Serratia marcescens biofilms grown on the surfaces demonstrated reduced biomass formation on the flattened surface after 5 days. Conditioning films on the surfaces of the bacteria, human plasma and human plasma combined with one of the bacteria demonstrated that the human plasma alone altered the surface properties the most, especially the roughness, surface chemistry and surface physicochemistry. When human plasma was combined with bacteria, changes were also determined but differed to when human plasma was tested alone. Finally, testing the effects of bacteria on platelets demonstrated that planktonic bacteria had little effect on platelet activation and viability, whilst biofilms halved platelet aggregation but did not affect activation. Western blots demonstrated that protein expression could be affected, particularly by biofilms. Further, release of RANTES by the platelets was doubled when incubated with bacterial biofilms. The results demonstrated the significance surface properties had on bacterial attachment and biofilm formation as well as how conditioning films altered the surface properties and how biofilms were able to affect platelets.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.799532  DOI: Not available
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