Use this URL to cite or link to this record in EThOS:
Title: Genetic and cellular mechanisms of autoimmunity in lupus-prone B6.129-Sle16 mice
Author: Buang, Norzawani Binti
ISNI:       0000 0004 8504 4220
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2017
Availability of Full Text:
Access from EThOS:
Access from Institution:
Genome sequencing and transcriptomic analyses are hypothesis-free approaches to identify genetic variants(s) and biological pathway(s) associated with a disease. T cells are paramount in the pathogenesis of systemic lupus erythematosus (SLE). Hyperactive CD4+ T cells can promote the production of auto-antibodies by offering excessive help to B cells. B6 congenic mice carrying the Sle16 locus (B6.129-Sle16) have increased auto-antibody levels, mild glomerulonephritis, and hyperactive T and B cells. These data indicate that intrinsic T cell abnormalities might drive the lupus-like disease development in B6.129-Sle16 mice. The aim of my thesis was to use exome sequencing and transcriptomic analysis of B6.129-Sle16 T cells from pre-disease mice to identify new candidate gene(s) and pathway(s) in lupus. Exome sequencing analysis uncovered 35 candidate genes with functional variants. RNAseq analysis of CD4+ and CD8+ T cells showed that the PYHIN gene family (Pydc4, Pydc3, Pyhin1 and Ifi204 genes) was the most differentially expressed in B6.129-Sle16 mice compared to B6 controls. PYHIN gene family expression is induced by type I interferon (IFN) and an increased IFN signature is a well-known characteristic in SLE. In my study, using in-vitro, in-vivo and ex-vivo analyses, I found no differences in the expression of IFN-inducible genes nor in pDC activation between B6.129-Sle16 mice and B6 controls. However, I observed that Pydc4 and Ifi204 gene expression could be modulated via anti-CD3 stimulation, indicating that these genes might directly regulate T cell proliferation, independent of type I IFN signalling. Altogether my findings identified new genes relevant to SLE and showed that the lupus-like disease in B6.129-Sle16 mice is independent of type I IFN, supporting the notion that lupus pathogenesis is driven by several mechanisms.
Supervisor: Botto, Marina ; Carlucci, Francesco Sponsor: Universiti Teknologi MARA
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral