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Title: The interaction between the 90 kiloDalton heat shock protein and steroid hormone receptors : The association of hsp90 and ER in the MCF-7 human breast cancer cell line (Part 1); The effect of temperature on the androgen responsiveness of two human prostate cancer cell lines (Part 2)
Author: Chalmers, Derek
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1991
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Breast cancer is a major killler of adult women, yet effective therapies are few. Prostate disease (both benign prostate hypertophy-BPH and prostate cancer) afflicts a large proportion of adult males. Both breast cancer, and prostatic disease, represent abnormal growth of reproductive cells. Epithelial cells of the male and female reproductive systems respond to a variety of stimuli, notably including the sex steroid hormones. These hormones exert their effect by binding to an intracellular receptor, or steroid receptor. Hormone sensitivity - the basis of successful endocrine therapy - is best defined, for breast cancer, as the presence of specific receptors for oestrogen and progesterone. Presence of functional steroid receptors may also reflect better prognoses. Steroid receptors are proteins that represent one category in the superfamily of intracellular receptors. They are composed of two types of protein subunits: steroid-/DNA-binding subunits and seemingly inert nonsteroid-/nonDNA-binding subunits. It is proposed that the two sets of subunits form multimeric complexes which appear as 'non-activated' steroid receptors. This large complex is unable to bind DNA. Binding of the correct steroid to the steroid-binding site of the steroid-/DNA-binding subunit(s) is thought to induce the dissociation of the inert subunit(s) to reveal the hitherto 'masked' DNA-binding sites. This process has been termed activation, and it is fundamental to productive hormone action. In recent years the 'inert' subunit has been the subject of much interest. This protein has been identified as the highly conserved 90 kiloDalton heat shock protein (hsp 90); a heat inducible protein that has also been found to associate with various components of the cytoskeleton. Since this protein was both a vital component of steroid receptors, and a member of the thermally-inducable heat shock protein family, the work reported in this thesis investigated whether the endocrine functions of hsp 90 could be enhanced or compromised after heat shock.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available