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Title: In vivo and in vitro studies of oral candidal colonisation and adhesion in diabetic subjects
Author: Darwazeh, Azmi Mohammad Ghaleb Fathallah
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1990
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Diabetes mellitus is the most common endocrine disorder affecting about one per cent of the population although about the same number are still undiagnosed clinically. It is believed that diabetic patients are more predisposed to infections than non-diabetic individuals and oral candidosis is more common in diabetic patients. Many local and systemic factors have been claimed to predispose an individual to oral candidal infection and the integrity of host defence factors seems to be an important determinant in mucocutaneous candidosis. Therefore, in Chapter One the local host defence factors of known importance, in addition to some general factors thought to increase the risk for oral candidosis have been reviewed, and particular attention has been directed toward those factors that might predispose diabetic patients to oral candidal infection. Since candidal adhesion to host surfaces is recognised as an essential prerequisite for colonisation and subsequent infection, factors affecting adhesion of candidal species to epithelial cell and denture acrylic surfaces, in addition to the possible mechanisms involved, were reviewed in Chapter Two. Chapter Three of this study aimed to study and compare the prevalence of candidal carriage and clinical candidal infection in the oral cavity of a group of diabetic patients and a matched group of control subjects using current methodology. In view of the results obtained and described in Chapter Three, the objectives of the study described in Chapter Four were to investigate and compare adhesion of C. albicans to buccal epithelial cells (BEC) in a group of diabetic patients and a closely matched group of non-diabetic individuals to determine whether a relationship existed between the in vitro candidal adhesion to BEC and the increased predisposition to clinical oral candidal colonisation and infection. In the light of the results of Chapter Four and the reports that pretreatment of acrylic strips with chlorhexidine gluconate reduced adherence of Candida species, the objective of Chapter Five was to investigate whether mouth rinsing with 0.2% chlorhexidine would similarly reduce candidal adhesion to BEC comparably in diabetic patients and non-diabetic individuals. The main aim of Chapter Six was to investigate whether nystatin, in therapeutic doses, would reduce candidal adhesion to buccal epithelial cells and whether any differences were discernable between diabetic patients and non-diabetic individuals.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available