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Title: AMP deaminase from Saccharomyces cerevisiae
Author: MacKenzie, Jane
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1989
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Attempts were made to clone the yeast adenosine-5'-monophosphate deaminase (AMP deaminase) gene from S. cerevisiae using a number of strategies. Initially, it was hoped that overproduction of AMP deaminase could be detected in yeast, by selecting for resistance to the AMP deaminase inhibitor, deoxycoformycin (dCF). This involved the construction of ade, hpt1 yeast strains in which the AMP deaminase reaction is the major source of inosine-5'-monophosphate (IMP). Although yeast AMP deaminase is sensitive to dCF in vitro no effect was observed in vivo (possibly due to lack of uptake of the drug).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available