Use this URL to cite or link to this record in EThOS:
Title: An investigation of the cellular stress response in cells infected with herpes simplex virus
Author: Notarianni, Elena
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1986
Availability of Full Text:
Access from EThOS:
Access from Institution:
This study entailed an investigation of the cellular stress response in secondary chick embryo fibroblasts (CEF) during infection by HSV. It was established that infections at a+ NPT with temperature-sensitive mutants of HSV-1 which are defective in immediate-early viral polypeptide Vmw IE 175 (i.e. tsD, tsK and tsT) cause the stress response to be induced, as manifest by a marked stimulation of synthesis of stress proteins. Induction by tsK was shown to be dependent upon the synthesis of immediate-early viral polypeptides. Infections with other mutants of HSV-1 (tsl201, tsB, tsE, tsG and MDK/2) at a NPT and, to a lesser extent, the revertant of tSK, ts+K, or with wt HSV-1 or wt HSV-2, all of which are non-defective in Vmw IE 175 or the HSV-2 equivalent, Vmw IE 182, cause synthesis of stress proteins to be increased. To account for these observations a hypothesis was advanced, that cells are subjected to stress during infection with wt or mutant HSV, owing to the expresssion of viral functions, and that induction of the stress response is subsequently inhibited depending upon the characteristics of the infecting virus: inhibition is most effective in cells infected with wt viruses, and least effective in cells infected at a NPT with temperature-sensitive mutants of HSV-1 defective in Vmw IE 175.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available