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Title: Molecular requirements and effector targeting of the internalisation of pattern recognition receptors mediating plant immunity
Author: Loiseau, Jenna
ISNI:       0000 0004 8501 8305
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2019
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In order to perceive pathogen-associated molecular pattern (PAMPs) derived from pathogenic microbes, plants express pattern recognition receptors (PRRs) at their cell surface, which mediate PAMP-triggered immunity (PTI). In Arabidopsis, bacterial PAMP flg22 perception undergoes internalisation of its cognate PRR FLAGELLIN-SENSING 2 (FLS2). Ligand-activated FLS2 follows the endocytic pathway through the late endosome/multivesicular bodies (LE /MVBs) compartments to the lytic vacuole for degradation. Advances have been made regarding our understanding of the subcellular trafficking of these receptors but the molecular mechanisms underlying the regulation of their trafficking and the interplay it has with immunity remain poorly understood. Recent data demonstrate that post translational modifications (PTM) regulate PRRs internalisation and is critical for the execution of immune responses. To better understand regulation of PRRs trafficking during immunity, I investigated regulation of PRRs subcellular trafficking upon PAMP perception by using a combination of live-cell imaging microscopy together with effector interference. Here, I present FLS2 traffics to the LE/MVBs via the trans-Golgi network (TGN)/early endosome (EE) and that this is dependent on the ADP RIBOSYLATION FACTOR GUANINE-NUCLEOTIDE EXCHANGE FACTOR (Arf-GEF) HopM1 interactor 7 (MIN7). Further, confirming that endocytosis is a common process among PRRs mediating immunity, I demonstrate that the Pseudomonas syringae effector HopM1 targets flg22-induced endocytosis of FLS2 and elf18-induced endocytosis of EFR at the TGN/EE but not constitutive endocytosis of BRI1. Additionally, I indicate that receptor activation is uncoupled from its internalisation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available