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Title: Identification of Rab32 interactors in Salmonella-infected cells and characterisation of the role of GtgE prenylation in Salmonella infection
Author: Yu, Hongjiao
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2019
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Salmonella enterica serovar Typhi is an intracellular bacterial pathogen that only infects humans. Remarkably, a BLOC-3 and Rab32-dependent anti-microbial pathway was identified to control the killing of S. Typhi in mouse macrophages and restrict S. Typhi infection in humans. This pathway can be counteracted by a broad-host Salmonella enterica serovar Typhimurium with its 2 effectors GtgE and SopD2. Yet very little is known about this novel pathway. Here, I used an explorative quantitative proteomics approach in combination with Rab32 pulldown assay and identified 3 proteins – RabGDIa, RabGDIß and Vps13C that specifically bind Rab32. With preliminary evidences achieved indicating a possible role of Vps13C and RabGDI in Salmonella infection, this study provides some clues for future investigation on discovering other components of this novel pathway. In addition to that, this study determined that a post-translational modification of GtgE leads to an increased virulence of S. Typhimurium during infection, which is likely via its increased ability to cleave Rab32.
Supervisor: Spano, Stefania Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Salmonella infections ; Proteomics ; Proteins