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Title: High resolution NMR based approaches for facilitating chromatography method development
Author: Dabo, Azzedine Abdelah
ISNI:       0000 0004 8498 0404
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2019
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Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) is one of the most widely used techniques for provision of analytical measurement information in the pharmaceutical industry from discovery through to development, clinical and manufacturing analytical laboratories. The pharma industry has been for many years attempting to transform the efficiency of method development and increasingly attention has turned to using in silico methodologies. Pfizer have been at the forefront of developing streamlined workflows and more recently, models for retention time prediction so as to reduce the amount of experimental effort in method development. Many of the in-silico strategies used rely on calculated parameters of the molecules under investigation and to some extent the characteristics of the RP-HPLC stationary phases, but no models yet have captured any measures that capture any description of the multiple and complex analyte - stationary phase-mobile phase interactions within any one RPHPLC method condition. To accomplish this, 1H high resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spin-lattice (T1) and spin-spin (T2) relaxation measurements were carried out to probe site-specific molecular motion of a series of aromatic compounds and reversed phase HPLC stationary phases in two different mobile phases. The NMR relaxation measurements provided insight about the nature of the site-specific interaction between the compounds with different stationary phases.
Supervisor: Not available Sponsor: Pfizer Ltd ; Engineering and Physical Sciences Research Council
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QC Physics ; QD Chemistry ; QP Physiology