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Title: The role of Feronia in regulating growth and development in Arabidopsis thaliana roots
Author: Goodman, Jennifer
ISNI:       0000 0004 8497 9032
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2018
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FERONIA (FER) is a receptor-like kinase (RLK) involved in a large number of processes in Arabidopsis. FER plays a role in cell elongation, mechanosensing, regulation of seed size, immunity and root hair development. A common theme in many of these processes is the involvement of FER as a regulator of location-specific growth (often called polar growth) and consistent with this observation FER is also known to interact with proteins that regulate the cytoskeleton (called Rho of plants, ROPs). The peptide, Rapid- Alkalinisation Factor 1 (RALF1), has been shown to elicit FER dependent signalling. Focusing within root tissue and using RALF1, we seek to explore early signalling events upon FER elicitation and clarify the interaction between FER and ROPs, using co-immunoprecipitation (CoIP) and mass spectrometry (MS). We have identified potential FER interactors, including Receptor for Activated C kinase 1B (RACK1B) and have identified two proteins, CAND1 and ILITYHIA, which are common between FER, ROP2 and ROP6. We also present data showing signalling events downstream of FER using a global phosphopeptide enrichment of Arabidopsis roots where we identified 684 phosphorylation sites dependent on RALF1 and/or FER.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QK Botany