Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.793902
Title: Control of developmental reprogramming during somatic cell regeneration in Arabidopsis thaliana
Author: Naish, Matthew
ISNI:       0000 0004 8497 7213
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2018
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Abstract:
Unlike most animals, plants can regenerate new tissues, organs or an entire organism by changing the developmentally committed program of individual cells - a phenomenon known as totipotency. This unique developmental plasticity of plants has been exploited in agriculture and horticulture to generate clonal plants in axenic culture through the induction of a somatic embryogenesis. However, some plant species are resistant to current regeneration protocols and the precise mechanisms responsible for totipotency in plant cells remain unknown. Using a novel system to induce somatic embryos from terminally differentiated somatic cells, we have investigated the importance of histone modifying proteins belonging to the Polycomb group (PcG) and Jumonji C-domain-containing class (JmJ-C), on cellular susceptibility to developmental reprogramming. We found that reprogramming success is influenced by cell type, developmental stage and that JmJ-C proteins play an important role in facilitating plant cellular plasticity. We also identified a protective role against epimutations during sexual reproduction in Arabidopsis thaliana. Furthermore, this system enabled the identification of key factors involved in the transition away from a differentiated state and into a new developmental fate, providing insights into the regulatory mechanism of totipotency in plants.
Supervisor: Not available Sponsor: Midlands Integrative Biosciences Training Partnership
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.793902  DOI: Not available
Keywords: QK Botany ; QR Microbiology
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