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Title: Exploring the molecular mechanisms of antimicrobial resistance in Brachyspira hyodysenteriae using whole genome sequencing
Author: Sheldon, Ewart Jonathan
ISNI:       0000 0004 8497 6288
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2018
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Brachyspira hyodysenteriae is the causative agent of swine dysentery, a disease characterised by bloody diarrhoea. It is endemic to the UK, and if untreated it can cause severe economic cost to farmers. Currently, it is treated with antibiotics including the pleuromutilin antibiotics tiamulin and valnemulin. B. hyodysenteriae has become more resistant to the antibiotics used to treat infections , and increasing levels of pleuromutilin resistance has been observed in some countries. In this study, 84 clinical isolates, from 2004 to 2015, were sequenced on an Illumina MiSeq. From this, the population structure of B. hyodysenteriae in the UK was constructed. In addition, the phenotypic resistance of 47 sequenced isolates was obtained using a commercial broth dilution assay. The use of sequenced isolates enabled detection of a recently identified pleuromutilin resistance gene and enabled prediction of the resistance phenotype of all sequenced isolates. The use of whole-genome sequencing has increased our knowledge of B. hyodysenteriae in the UK, highlighting potential regional differences and has created a reference database of all B. hyodysenteriae isolates from 2004 to 2015. This will improve surveillance and increase the power of outbreak analysis. Direct sequencing from clinical samples could further strengthen outbreak analysis and surveillance. This would improve the speed of identification and could provide useful information. To explore swine dysentery, positive samples were sequenced on an Illumina MiSeq and B. hyodysenteriae reads were extracted. From this, it was possible to identify the closest sequenced strains. This study highlights the potential uses of whole genome sequencing to analyse B. hyodysenteriae.
Supervisor: Not available Sponsor: Veterinary Medicines Directorate ; Warwick Medical School
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: SF Animal culture