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Title: Vaccine potential of Adhesin Complex Protein (ACP) from Neisseria gonorrhoeae
Author: Almonacid Mendoza, Hannia Liliana
ISNI:       0000 0004 8501 796X
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 2017
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Sexual transmitted disease Gonorrhoea is caused by the organism Neisseria gonorrhoeae, infecting ~106 million cases annually. Currently, a lack of an effective vaccine against this pathogen and treatments using last generation of antibiotics are misleading due to emerging antibiotic-resistant superbugs. Recently has been described a small protein in a closed related bacteria, N. meningitidis (Nm), termed as an Adhesin Complex Protein (ACP). Nm-ACP is an outer-membrane protein which is a conserved protein in commensal and pathogenic bacteria. Besides Nm-ACP is capable of induce cross-protective bactericidal antibodies. A homologue gene, ng-acp (NGO1981) from Neisseria gonorrhoeae strain P9-17 is highly conserved among gonococcal isolates reported until the date. The ng-acp gene product was cloned into pRSET-A and pET-22b cloning vector systems and expressed as a recombinant protein in E. coli BL21pLysS to be used in immunization trials in murine model using a range of adjuvants and delivery formulations. Raised mice serum demonstrated a great reactivity against recombinant rNg-ACP by ELISA and displayed cross-strain reactivity in gonococcal outer-membrane (OMV) and lysate preparations from N. gonorrhoeae strains P9-17 and FA1090 by western-blot. Antisera r-Ng-ACP showed high bactericidal properties against homologous and heterologous wild type strains compared to the knockout strains. Furthermore, Ng-ACP plays a role of association on different epithelial cells showing a reduction ̴75-50% by comparison the wild-type and knockout. Three-dimensional structure of rNgACP the overall fold resemble of the lysozyme inhibitors from Salmonella typhimurium (PliC family) and recently described Neisseria meningitidis.Taken all together, suggest that Ng-ACP from N. gonorrhoeae is a potential candidate to develop an anti-gonococcal vaccine.
Supervisor: Christodoulides, Myron ; Clarke, Ian ; McCormick, C. Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available