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Title: Antimicrobial screening of secondary metabolites from Solanaceae
Author: Nice, Katarina Jane
Awarding Body: Royal Holloway, University of London
Current Institution: Royal Holloway, University of London
Date of Award: 2013
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The aim of the project was to analyse the potential antimicrobial activity of extracted secondary metabolites from wild tomato species, and other Solanaceous plants. The wild tomato species such as Solanum lycopersicum cv M82, Solanum cheesmaniae, Solanum chmielewskii, Solanum chilense, Solanum peruvianum, Solanum pimpinellifolium, Solanum pennellii, Solanum habrochaites, and Solanum neorickii were the primary focus of the project which were analysed for antimicrobial compounds. The hypothesis that the wild tomato relatives could be antimicrobial is linked to the insecticidal and fungicidal properties of several wild tomato relatives. This research is further supported by the previous findings into the antimicrobial potential of certain Solanaceae species, where the leaf tissue was noted to have the highest general antimicrobial activity. As part of this project the fully expanded undamaged leaves were examined for antimicrobial activity, after extracting the secondary metabolites in a range of solvents. Antimicrobial activity was determined through monitoring the plant extracts effect on the growth of gastroenteric bacteria Salmonella, Escherichia coli, and Staphylococcus aureus primarily through disc diffusion assays and growth curve analysis. The metabolomic profile of Staphylococcus aureus grown in the presence of identified antimicrobial Solanum pennellii extract was used to identify the compounds mode of action, and electron microscopy was used to view the effects of this extract upon the bacteria cell structure. Further putative identification of selected antimicrobial extracts was achieved through the use of thin layer chromatography, and gas chromatrography-mass spectrometry analysis. The Solanaceae plants which were identified to have a significant antimicrobial activity were Solanum habrochaites, Solanum pennellii, and Nicotiana rustica. The trichomes of these plants were identified as the primary target site for the production of these antimicrobial compounds.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available