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Title: Development of an in vitro gingivitis model
Author: Wiecek, J. M.
ISNI:       0000 0004 8503 0750
Awarding Body: UCL (University College London)
Current Institution: University College London (University of London)
Date of Award: 2015
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Gingivitis a gum disease, affects 50-90% of the adult population worldwide, if left untreated gingivitis can lead to periodontitis. However, even though gingivitis is highly prevalent, the pathogenicity of the disease is still poorly understood; highlighting the need for a reliable and reproducible in vitro gingivitis model which could help elucidate clinically important questions and provide a better understanding of gingivitis aetiology. In this study the original Constant Depth Film Fermentor (CDFF) was modified to create a Triple-CDFF (T-CDFF) allowing concurrent growth of oral biofilms that can be treated separately in a controlled, flexible environment. Several mechanical changes were implemented to increase the model's reliability, reproducibility and manoeuvrability; including improving air-tightness of the model by applying better seals, re-shaping model parts to increase portability. A standardised experimental methodology was devised; this included sampling procedures to allow reliable and reproducible growth of oral biofilms across the individual units of this system. Biofilms obtained from T-CDFF under health and disease conditions were screened for eight bacteria; using qPCR primers for S. sanguinis, V. dispar, N. subflava, S. mutans, L.casei, F. nucleatum, P. intermedia, and A. naeslundii. To investigate the presence of other bacteria associated with gingivitis such as T. denticola, P. gingivalis, a trypsin-like-protease assay was applied. Next generation sequencing combined with metabolomics gave a better understanding of the bacterial changes occurring during simulated disease progression. In conclusion, this study has successfully (i) developed and validated a new complex in vitro T-CDFF system and also (ii) showed great potential for modelling gingivitis in vitro; although further verification needed. The system was considered reliable and shows a great potential for being used as a standardised model for testing dentifrices and antimicrobials.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available