Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.786767
Title: Expanding the scope of electron paramagnetic resonance spectroscopy for structural studies of proteins and peptides
Author: Shah, Anokhi
ISNI:       0000 0004 7972 2056
Awarding Body: University of St Andrews
Current Institution: University of St Andrews
Date of Award: 2019
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Abstract:
Electron paramagnetic resonance spectroscopy is a versatile tool for probing structural information about systems with unpaired electrons, in particular, biological systems with metal centres or chemically attached spin labels. This work uses a variety of electron paramagnetic resonance techniques to investigate the ability to measure distances between two spins (radical and metal), as well as characterise the local environment. Distance measurements are used to probe the mechanism of translocation across a membrane, where a change in distance is observed upon ATP cycling, indicating channel movement of the SecYEG:SecA complex. Furthermore, to expand the scope of distance measurements for more complex, cysteine-rich systems, spin labelling regimes are developed and optimised on the test protein myoglobin. Specifically, next generation maleimide spin labels are demonstrated to label and selectively cleave. Myoglobin is further exploited to successfully introduce the unnatural amino acid, dehydroalanine, for selective and orthogonal labelling. The development of the labelling and measurement strategy for the gadolinium-based spin label, [Gd.sTPATCN]-SL, is also shown, where the narrow central transition of the label allows a long phase memory time and increased DEER modulation depth, to give increased measurement sensitivity. In addition, gadolinium(III) distances are used to characterise the binding site of a peptide system for the application of magnetic resonance imaging. The optimisation of measuring inter-gadolinium(III) distances between 2-5 nm at both Q- and W-band is also demonstrated in the corresponding peptide ruler series. The additional benefit of the peptide to act as a metal ruler is further investigated using copper(II), where hyperfine spectroscopy is utilised to successfully confirm the nature of the binding site as all oxygen binding.
Supervisor: Lovett, Janet Eleanor Sponsor: Engineering and Physical Sciences Research Council (EPSRC)
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.786767  DOI: Not available
Keywords: Electron paramagnetic resonance ; EPR ; Mass spectrometry ; Spin labelling ; New labels ; Structural biology ; Metals ; Copper ; Gadolinium ; Membrane protein ; Protein ; Peptide ; Distances ; DEER ; QC763.S5 ; Electron paramagnetic resonance spectroscopy ; Molecular biology ; Molecular structure
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