Over half of the UK population over 75 suffers from osteoarthritis (OA). It is the most common musculoskeletal disorder, with pain presenting as the most prevalent symptom. Three quarters of patients with OA complain of pain on a daily basis, often severely affecting quality of life. Understanding the mechanism behind the generation of pain in OA, with a view to abrogate this symptom, is of utmost clinical importance. The results of this thesis explore unchartered territory of pain research in OA. It provides further insights into the pathogenesis of pain in this disease, and is likely to have translational benefits into clinical medicine in the future.