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Title: An investigation into the effects of substrate properties on the mechanics of corneal epithelial cells
Author: Holland, Preeti
ISNI:       0000 0004 7970 8895
Awarding Body: Loughborough University
Current Institution: Loughborough University
Date of Award: 2018
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Cells respond to mechanical changes in their extracellular environment, as reflected by various cell behaviours and observed through changes in the tissue biomechanics. Types of cell behaviour that are regulated by mechanical cues in the microenvironment of the cell are cell spreading, migration, proliferation and differentiation. Cell migration is a key part of many biological processes including corneal wound repair. Changes in the biomechanical properties of the cornea can be induced by refractive and therapeutic treatments and also by diseases of the eye or other illnesses. A Rabbit Corneal Epithelial (RCE) cell line was used to study cell mechanics and cell migration. Polydimethylsiloxane (PDMS), a biocompatible silicone elastomer, was used as a substrate to culture RCE cells. In order to promote cell attachment and growth, the hydrophilicity of the PDMS surface was increased by treating it with oxygen-rich cold atmospheric pressure plasma, which was confirmed by surface characterisation techniques. Cell attachment and growth studies over time comparing plasma and non-plasma treated PDMS showed an increase in RCE cell growth and area coverage on plasma treated PDMS.
Supervisor: Not available Sponsor: Loughborough University
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Mechanical Engineering not elsewhere classified ; Corneal epithelial cells ; PDMS ; Substrate stiffness ; Mechanical properties ; AFM ; Single-cell measurements