Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.783678
Title: Anatomical and functional investigation of the deep medial entorhinal cortex
Author: Gerlei, Klára Zsófia
ISNI:       0000 0004 7969 2627
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2019
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Abstract:
Spatial computations are thought to be processed in the hippocampal-entorhinal network and involve neurons with spatially selective firing properties. The deep medial entorhinal cortex (MEC) consists of molecularly distinct sublayers with distinct connectivity. One of these layers, layer 5b (L5b) receives input both from the hippocampal cornu ammonis 1 (CA1) area and the superficial MEC, regions that have abundant spatially selective cells. Extracellular recordings from rats found grid and conjunctive cells in the deep MEC, while other studies suggest that deep MEC neurons are silent. Based on its position in the circuit and the presence of spatial cells, I hypothesized that L5b plays a role in spatial cognition. To test this hypothesis, I first investigated approaches to target L5b cells with genetic precision and characterized transgenic mouse lines. I found a mouse line that had tTA expression sufficiently specific to L5b, and used this line to investigate the anatomy and firing properties of L5b cells. I developed an analysis pipeline to automate the analysis of extracellular recordings using MountainSort, and evaluated opto-tagging of genetically defined cells in an open field arena. I successfully opto-tagged one genetically defined deep layer cell and a small number of cells that receive input from genetically defined deep layer cells. My analysis of the firing properties of deep MEC cells recorded from mice indicates that grid and head-direction properties are similar to what was reported in the rat. My results support the idea that deep layers of MEC have a powerful influence on more superficial layers and suggest how cells in this area may contribute to spatial behaviours.
Supervisor: Nolan, Matthew ; Wood, Emma Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.783678  DOI: Not available
Keywords: spatial navigation ; deep entorhinal cortex ; in vivo electrophysiology
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