Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.783587
Title: Molecular epidemiology of multi-drug resistant Escherichia coli in companion animal species
Author: Wagner, Samuel Jack Alexander
ISNI:       0000 0004 7969 1720
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2019
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
This study focused on a group of multi-drug resistant (MDR) E. coli collected between 2002 and 2011 at the Small Animal Hospital, Royal (Dick) Veterinary School; isolated from urinary tract infections in dogs. The MDR E. coli isolates were compared against a group of fully susceptible E. coli, also collected from canine urinary tract infections. The MDR isolates were notable for their AmpC β-lactamase resistance phenotype (12/18) and resistance genotype (blaCMY-2, 8/12). Phylogenetic comparisons between the MDR and susceptible groups of isolates showed a large degree of separation between the two groups. Susceptible isolates strongly associated the the pathogenic B2 phylogroup (67%), which was expected. The MDR group was much more mixed, with isolates more associated with commensal A (28%) and B1 (22%) phylogroups. Virulence marker abundance, evaluated using an Identibac microarray, showed a much reduced presence of virulence markers in the MDR group, as compared with the susceptible isolates. An infection model, using Galleria mellonella larvae, was used to better test the phenotypic virulence of the MDR and susceptible E. coli isolates. An asymptomatic bacteriurea strain (ABU83972) and a virulent pyelonephritis strain (CFT073) were used as non-virulent and virulent control strains of uropathogenic E. coli. The model produced consistent and expected differences in lethality between ABU8392 (non-lethal) and CFT073 (lethal) strains. However, the model showed no differences between the MDR and susceptible isolates. Second generation Illumina short-read sequencing and third generation single-molecule real-time sequencing were used to further dissect the genetic background of both the E. coli and the MDR genotype. Core-genomic sequence comparisons of the E. coli showed no clonal relatedness amongst the different MDR and susceptible strains. Most strikingly, sequencing revealed extensive plasmid carriage of the MDR genotype. Plasmid mediated blaCMY-2 was associated with a clonal group of IncI1 plasmids. The IncI1 plasmids show favorable sequence comparisons to a plasmid backbone previously reported in dogs and other animals, with a global distribution. The remaining resistance genes were associated with a group of IncFII plasmid sequences. This study highlights a broader range of commensal E. coli, which have resulted in opportunistic infections in their canine hosts. As such, they act as a potential reservoir of resistance, which is as yet uncharacterized.
Supervisor: Argyle, Sally ; Gally, David Sponsor: Biotechnology and Biological Sciences Research Council (BBSRC)
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.783587  DOI: Not available
Keywords: antibiotic resistance ; E. coli ; canine ; plasmids ; AmpC ß-lactamase resistance phenotype ; MDR genotype ; reservoir of resistance
Share: