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Title: Population and functional genomic analysis of Lawsonia intracellularis
Author: Bengtsson, Rebecca Ji
ISNI:       0000 0004 7969 1165
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2019
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The Gram-negative, obligate intracellular bacterium Lawsonia intracellularis is the aetiological agent of a non-zoonotic enteric disease known as proliferative enteropathy (PE). PE has been detected in wide range of mammalian species, including wild and domestic animals. The disease is most prevalent in pig herds where it is endemic, with both subclinical and clinical infections. Porcine PE was first described in 1931 and four different form of the disease are recognised, ranging from mild to severe clinical manifestations. Over recent years rising outbreaks of equine PE in foals have been reported and cross-species experimental infections have demonstrated host-specificity for infection. Although the dynamics of L. intracellularis infection have been examined, there is a lack of understanding population genetic structure and the basis for pathogenesis due to difficulties with culturing obligate intracellular organisms. In this study, we developed a pipeline to obtain high quality whole genome sequences of L. intracellularis through direct sequencing of clinical samples from field outbreaks of porcine and equine PE, and cell passaged samples without the requirement of cultivation, resulting in the generation of 25 draft genome assemblies. To explore the genetic diversity of L. intracellularis, we performed comparative analysis which revealed a clonal population structure among porcine derived isolates showing very limited diversity, indicative of emergence from a recent clonal expansion. Our analysis revealed that infections among different hosts are caused by genetically distinct L. intracellularis sub-types. Finally, we focussed on a core gene encoding a putative autotransporter, termed Lawsonia autotransporter B (latB), that exhibited genetic variation in different strains, and immunofluorescent staining for LatB revealed the putative autotransporter was expressed during infection and was associated with the bacterial outer membrane. Overall, these studies have provided insights into the genetic diversity and evolutionary origin of L. intracellularis, enhancing our understanding of the population biology of this important animal pathogen.
Supervisor: Fitzgerald, Jonathan ; Ait-Ali, Tahar Sponsor: Biotechnology and Biological Sciences Research Council (BBSRC)
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Lawsonia intracellularis ; proliferative enteropathy ; low levels of genetic diversity