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Title: The development of sea sponges (Hymeniacidon perlevis and Amorphinopsis sp) as novel models for genotoxicity assessment and environmental monitoring of pollutants in the aquatic environment
Author: Akpiri, Rachael Ununuma
ISNI:       0000 0004 7967 9895
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2019
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The measurement of chemical levels of pollutants and their Biomarkers in resident sessile biota has been suggested for reliable aquatic monitoring and environmental risk assessment. This thesis proposes the use of the comet assay in sponges as a suitable model to complement analytical methods and other established invertebrate aquatic sentinels in aquatic pollution monitoring and risk assessment. A novel 'in vivo' exposure sponge cell model was optimised for assessment of DNA strand breaks (DSB) using the alkaline comet assay and Reactive oxygen species (ROS) formation using 2',7'-Dichlorodihydrofluorescein diacetate (H2DCF-DA) assay. Concentration dependent increase in DSB and ROS formation were observed in sponge cells exposed to aluminium, cadmium, chromium, and nickel in lab based toxicity testing. Correlation between DSB and ROS formation decreased in the order Al< Cd
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QH426 Genetics ; TD Environmental technology. Sanitary engineering