| Use this URL to cite or link to this record in EThOS: | https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.782384 |
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| Title: | The development of sea sponges (Hymeniacidon perlevis and Amorphinopsis sp) as novel models for genotoxicity assessment and environmental monitoring of pollutants in the aquatic environment | ||||||
| Author: | Akpiri, Rachael Ununuma |
ISNI:
0000 0004 7967 9895
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| Awarding Body: | University of Birmingham | ||||||
| Current Institution: | University of Birmingham | ||||||
| Date of Award: | 2019 | ||||||
| Availability of Full Text: |
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| Abstract: | |||||||
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The measurement of chemical levels of pollutants and their Biomarkers in resident sessile biota has been suggested for reliable aquatic monitoring and environmental risk assessment. This thesis proposes the use of the comet assay in sponges as a suitable model to complement analytical methods and other established invertebrate aquatic sentinels in aquatic pollution monitoring and risk assessment. A novel 'in vivo' exposure sponge cell model was optimised for assessment of DNA strand breaks (DSB) using the alkaline comet assay and Reactive oxygen species (ROS) formation using 2',7'-Dichlorodihydrofluorescein diacetate (H2DCF-DA) assay. Concentration dependent increase in DSB and ROS formation were observed in sponge cells exposed to aluminium, cadmium, chromium, and nickel in lab based toxicity testing. Correlation between DSB and ROS formation decreased in the order Al< Cd |
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| Supervisor: | Not available | Sponsor: | Not available | ||||
| Qualification Name: | Thesis (Ph.D.) | Qualification Level: | Doctoral | ||||
| EThOS ID: | uk.bl.ethos.782384 | DOI: | Not available | ||||
| Keywords: | QH426 Genetics ; TD Environmental technology. Sanitary engineering | ||||||
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