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Title: Signal crosstalk between calcium-sensing and prostanoid receptors and its role in parathyroid hormone secretion
Author: Albar, Halah
ISNI:       0000 0004 7965 2924
Awarding Body: University of Manchester
Current Institution: University of Manchester
Date of Award: 2018
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The calcium-sensing receptor (CaR) maintains extracellular calcium Ca2+o homeostasis by suppressing parathyroid hormone (PTH) secretion. However, the underlying cellular driving force for tonic PTH secretion remains unclear. It is believed that agonists that generate intracellular cyclic-AMP levels may increase PTH secretion and therefore bidirectional crosstalk between CaR signalling and cAMP levels could be of physiological importance. In order to identify possible contributors to parathyroid cAMP accumulation I first confirmed the gene and protein expression of EP4R (prostanoid) and CALCRL (calcitonin receptor-like receptor) in bovine PT gland by using RT-PCR and western blotting respectively. Also, I confirmed the gene expression of the PGE synthase enzyme required for the PGE2 synthesis in bovine PT gland by RT-PCR. And I further confirmed the gene expression of the CALCRL's associated components particularly the RAMPs and adrenomedullin.
Supervisor: Ward, Donald ; Dunne, Mark Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: prostanoid ; calcium ; parathyroid hormone ; secretion ; intrinsic mechanism