Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.777446
Title: Studies on the development, maturation and radiosensitivity of the mammalian ovary
Author: Baker, Terry G.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1975
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Abstract:
The thesis is subdivided into four closely related sections, although the various studies were carried out concurrently and form an integrated and on-going research programme. Section I deals with studies on:- (i) the development of the ovary; (ii) quantitative histological and cytological studies of oogenesis; (iii) electron microscope studies of oogonia and oocytes, and the origin of cytoplasmic organelles; (iv) the significance of lampbrush chromosomes in mammalian oocytes at diplotene; and (v) the control of oogenesis in fetal ovaries, and in Graafian follicles. Section II of the thesis is concerned with the radiosensitivity of oogonia and oocytes in human and rhesus monkey ovaries (using the criteria of cell death and reproductive capacity). The results of these studies are reviewed in the light of information on: (a) other species, . and b) other criteria of radiosensitivity. The published material also includes observations on the response to X-irradiation of oogonia and oocytes in organ culture (particularly human ovaries), and on the fine-structure of germ cells undergoing radiation-induced degeneration. An hypothesis is discussed which accounts for the marked interspecies variation in response of oocyfes at the diplotene/dictyate stage of meiosis. Section III is concerned with the endocrine control of=: pre-ovulatory maturation in Graafian follicles, both in vivo and in organ culture. The results confirm the importance in these processes of the so-called 'LH-surge', but also show that prostaglandins and FSH can.induce oocyte maturation and progesterone synthesis in organ culture. Indeed, lower doses of FSH than of LH are required to cause the resumption of meiosis in oocytes, although the reverse is true for progesterone synthesis. Section IV contains three papers. The first describes a possible case.of superfetation in man, while the second describes ultrastructural changes which occur in the rat thyroid gland maintained in organ culture. The final publication confirms the view that the secretion of testosterone by the fetal human testis maintained in culture is greatly enhanced by the hCG. The thesis is subdivided into four closely related sections, although the various studies were carried out concurrently and form an integrated and on-going research programme. Section I deals with studies on:- (i) the development of the ovary; (ii) quantitative histological and cytological studies of oogenesis; (iii) electron microscope studies of oogonia and oocytes, and the origin of cytoplasmic organelles; (iv) the significance of lampbrush chromosomes in mammalian oocytes at diplotene; and (v) the control of oogenesis in fetal ovaries, and in Graafian follicles. Section II of the thesis is concerned with the radiosensitivity of oogonia and oocytes in human and rhesus monkey ovaries (using the criteria of cell death and reproductive capacity). The results of these studies are reviewed in the light of information on: (a) other species, . and b) other criteria of radiosensitivity. The published material also includes observations on the response to X-irradiation of oogonia and oocytes in organ culture (particularly human ovaries), and on the fine-structure of germ cells undergoing radiation-induced degeneration. An hypothesis is discussed which accounts for the marked interspecies variation in response of oocyfes at the diplotene/dictyate stage of meiosis. Section III is concerned with the endocrine control of=: pre-ovulatory maturation in Graafian follicles, both in vivo and in organ culture. The results confirm the importance in these processes of the so-called 'LH-surge', but also show that prostaglandins and FSH can.induce oocyte maturation and progesterone synthesis in organ culture. Indeed, lower doses of FSH than of LH are required to cause the resumption of meiosis in oocytes, although the reverse is true for progesterone synthesis. Section IV contains three papers. The first describes a possible case.of superfetation in man, while the second describes ultrastructural changes which occur in the rat thyroid gland maintained in organ culture. The final publication confirms the view that the secretion of testosterone by the fetal human testis maintained in culture is greatly enhanced by the hCG.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (D.Sc.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.777446  DOI: Not available
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