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Title: Contribution of the electron microscope to molecular biology
Author: Harris, James R.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1981
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This thesis contains several published works, together with seven presently unpublished articles, most of which will, in due course, be published. A correlative essay serves to put the overall subject matter in perspective, drawing on my own work and the work of others. The areas covered in the thesis are of necessity very restricted, as it is impossible to survey in entirity the many fields in which electron microscopy has made a contribution to Molecular Biology. In this context the term Molecular Biology is used in the broadest sense of its meaning. An account of the preparation of nuclear envelope 'ghosts' subsequent to nuclear swelling leads on to a review of the ultra-structure and biochemistry of the nuclear envelope and the nuclear pore complex. This is followed by a piece of research on the protein apoferritin and oligomeric groups which occur naturally. A review is presented on non-enzymic proteins, which emphasises protein ultrastructure, derived in the main by negative staining. A short piece of research describes the application of the negative staining - carbon film technique, of Horne and his colleagues, to purified protein molecules. This work shows that regular monomolecular layer arrays of proteins can be produced, but that problems are very often encountered. A survey is presented of the current state of knowledge of the two erythrocyte proteins now termed Torin and Cylindrin. Theseproteins were discovered by electron microscopy during the studies of the candidate for the degree of Ph.D., and information on their characterization has slowly accumulated over the years since then. Very recently, progress has been made on. the possible enzymic activity of the protein Cylindrin, -but this is not included in this thesis. Strong evidence is accumulating that Cylindrin is an aminoacyl tRNA synthetase. Each unpublished submission is a complete article, including its own reference material. This has inevitably lead to some repetition of content in different sections, but it is hoped that this does not detract from the overall balance and content of the thesis. The published works submitted with the thesis fall into three categories: (A) Studies on erythrocyte membrane, hepatoma and cultured cell proteins; (B) Studies on avian erythrocytes and (C) Studies on rat liver nuclear envelope.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (D.Sc.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available