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Title: The purification of soya lipoxidase
Author: Cameron, Mary Davidson
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1957
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During a study of the oxygen uptake of wheat-flour doughs, it was found difficult to distinguish between reactions catalysed by lipoxidase and similar reactions catalysed by haematin compounds. All these systems are capable of catalysing the oxidation of unsaturated fats and may also cause secondary bleaching of carotenoids and other pigments. Valid comparisons between the two systems required the preparation of lipoxidase in a purified form and this jpurification was the purpose of the present work. The isolation of lipoxidase has been achieved in only one recorded case, by a complex combination of precipitive and electrophoretic methods. This thesis describos the application of the use of ethanol as a precipitant in a purification procedure for lipoxidase. A study of precipitation conditions is reported, and arising from the data obtained, routes of separation are described on an experimental basis. From these studies and the experience accumulated a simple procedure for obtaining a useful degree of concentration of the enzyme is described.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available