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Title: Cell surface changes induced by vaccinia virus
Author: Liddell, Kenneth G.
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1978
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Virus-specified antigens induced in the membranes of infected cells have been implicated as primary targets in the recognition and destruction of virus-infected cells by the immune system of mammalian hosts. Antigenic changes induced in the plasma membranes of vaccinia-infected HeLa cells 2 h post-infection were detected by immunofluorescence, immune haemadsorption, and to a lesser extent by complement-fixation and complement-mediated cytolysis. Cytopathic effects (cell rounding) were seen prior to viral DM replication and also when this was blocked. Other changes occurred at the cell surface later in infection when turkey erythrocytes and concanavalin A were bound by cells. There was no evidence for an overall increase in mucopolysaccharide synthesis in the form of sialic acid. Quantitative spectrophotometric assays for haemadsorption, immune haemadsorption and concanavalin A binding were developed and the effect of varying growth or reaction conditions on the expression of vaccinia haemagglutinin at the surface of infected cells was investigated. Vaccinia cell surface haemagglutinin (VHA), detected by the binding of turkey erythrocytes, was synthesised around 10 h post-infection, even under conditions of low multiplicity of infection, and was closely coincident with the appearance of infectious virus. Of seven different cell lines tested, haemadsorption was greatest in HeLa cells and least in L-929 cells. Other red cell species were bound to infected cells to a lesser degree than turkey cells.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available