Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.776306
Title: Steroid hormone and growth factor action in normal and cancer cells
Author: Munir, Mohammad Idrees
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1983
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Abstract:
The endometrium is an epithelio-mesenchymal structure. The growth and differentiation of normal endometrial cells is regulated by the interactions of both polypeptide and steroid hormones. An understanding of how hormones affect proliferation and metabolic activities, necessarily involves knowledge of the regulatory processes of these cellular functions. The regulation of the frequency of replication in animal cells is a complex phenomenon for which much of the molecular basis is currently unknown. Cell cultures, which are exempt from tissue and systemic influences are ideal for investigating whether individual growth factors are acting directly on the endometrial cell. Primary cultures of both normal and malignant epithelial cells were investigated. These cells have been grown both as pure epithelial cultures and as mixed populations of epithelial and stromal cells. The purpose of this study was first, to evaluate some of the critical data now available on various aspects of steroid regulated growth, and second to develop in vitro new models of steroid promoted growth that can accommodate current information. Different cell types, from both rat uterus and human endometrium, were separated by enzymatic and mechanical techniques. Epithelial cells were tentatively identified by comparison of their morphologic features in culture with the well documented features of endometrial cells in vivo. Moreover, epithelial nature was also confirmed using immunocytochemical criteria. Growth dynamics of these cells in culture were analysed by the classical techniques of 3H-thymidine incorporation, cell proliferation index and morphological criteria. Serum performs many functions in cell culture. In addition to providing classical hormones and growth factors, it compensates for the deficiences of defined media, supplying additional nutrients and trace elements. To determine the minimum requirements for foetal calf serum in cultures of normal and malignant cells derived from endometrium, this study reports a detailed examination of growth requirements of endometrial cells during the early culture. The results presented in Chapter 4 strongly suggest that it is possible to eliminate serum from culture medium and that the main function of serum in cell culture is to furnish hormones and other growth factors. The most important factors are EGF, transferrin, insulin and glucocorticoids. The role of serum in attachment and spreading of these cells was also assessed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.776306  DOI: Not available
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