Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775608
Title: Synthesis of β-lactone, γ-lactam 20S proteasome inhibitors
Author: Goodyear, Ross
ISNI:       0000 0004 7962 7833
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2019
Availability of Full Text:
Access from EThOS:
Access from Institution:
Abstract:
The 20S proteasome is a large protein complex, primarily responsible for the breakdown of ubiquitinated proteins. It is therefore a vital component in the ubiquitin proteasome pathway, disruptions of which can induce cell death. Such an effect could be targeted for cancer therapeutics. Some β-lactone γ-lactam scaffolds, such as the natural product omuralide (the biologically active form of lactacystin) have been found to inhibit the 20S proteasome. The discovery of several new natural 20S proteasome inhibitors such as the salinosporamides and cinnabaramides, have shown potential for the development of new inhibitors with increased potency and specificity. Development of synthetic routes to produce these targets and their analogues is key to developing new proteasome inhibitors with greater potency and reduced side effects. [Figure 1. Several natural products with 20S proteasome inhibitory activity] Our methodology involves the incorporation of amino acids into the γ-lactam core. Leucine and serine have been used to produce a new formal synthesis of 9-deoxy omuralide and omuralide respectively. Key steps include a diastereoselective acylation with Mander's reagent and desulfurization. This methodology offers a flexible route allowing rapid generation of omuralide analogues in order to produce known and novel 20S proteasome inhibitors for biological testing. [Figure 2. An overview of our methodology] In addition to our work incorporating amino acids into γ-lactam cores, we have also developed a new route to synthesize either diastereomer of hydroxy leucine. We hope that this valuable intermediate could be incorporated into the γ-lactam using our devised methodology quickly forming an advanced intermediate of omuralide.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.775608  DOI: Not available
Share: