Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775145
Title: Genome skimming for phylogenomics
Author: Dodsworth, Steven Andrew
ISNI:       0000 0004 7962 3410
Awarding Body: Queen Mary, University of London
Current Institution: Queen Mary, University of London
Date of Award: 2015
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Abstract:
The advent of next-generation (or high-throughput) sequencing (NGS/HTS) has revolutionised biology, with much impact on the field of molecular phylogenetics. Traditional debates of taxa versus characters are now somewhat defunct in the phylogenomics era. In this thesis I focus on one particular HTS approach, 'genome skimming' as a phylogenomics and genomics method. I extend the scope of genome skimming to encompass more of the data present from low-coverage genome sequencing, using a novel method to analyse genomic repeat abundances as phylogenetic characters in addition to the assembly of high-copy organellar and nuclear DNA (plastomes and the nuclear ribosomal DNA cistron). The methodology for using nuclear repeats is initially developed, and then genome skimming is used to explore the phylogenetic relationships within a recent radiation - Nicotiana section Suaveolentes (Solanaceae). These data provide a significant improvement in our phylogenetic understanding of the group, despite low levels of genetic divergence between the core Australian species of Nicotiana section Suaveolentes and significant incomplete lineage sorting. Support is garnered for the whole genome duplication (WGD) radiation lag-time model in section Suaveolentes, with a significant increase in diversification in the last 2 million years following a lag of approximately 4 million years after the origin of the section at ~6.8 mya (allopolyploidisation event). Associated with this diversification are various processes of diploidisation including chromosome number reduction and genome downsizing. In addition to genomic patterns, there are ecological ones associated with diversification, including a general switch from perennial to annual life history strategy (with some notable reversals). These results paint Nicotiana section Suaveolentes as a recent and ongoing radiation, and are placed in the broad context of angiosperm diversification post-polyploidisation.
Supervisor: Not available Sponsor: NERC ; Linnean Society ; Systematics Association ; Botanical Research Fund
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.775145  DOI: Not available
Keywords: Biological and Chemical Sciences ; next-generation sequencing ; molecular phylogenetics
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