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Title: Development of an alginate based microcarrier for cell expansion
Author: Chui, Chih-Yao
ISNI:       0000 0004 7960 0702
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 2019
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Mesenchymal stem cells (MSCs) are potential therapeutic candidates, owing to their differential ability. However, the gap between availability and demand of MSCs requires alternative expansion methods from 2D flasks such as microcarriers which provide a high surface area to volume ratio. However, current commercial microcarriers support low cell attachment and difficulty in cell detachment. This study developed genipin crosslinked alginate-chitosan microcarriers to overcome the aforementioned issues with commercial microcarriers. Alginate beads produced by electrospraying were coated with chitosan and crosslinked in genipin. The degree of crosslinking was determined through fluorescence of genipin-chitosan conjugates. By implementing a high crosslinking temperature of 60°C compared to the traditional 37°C, the microcarrier production time was significantly decreased. To ensure microcarrier stability under cell culture conditions, atomic force microscopy (AFM) based indentation assessing the local elastic reduced modulus (E*) was performed in parallel to measurement of bead swelling. Results generally show stability of E* and diameter of microcarriers. Additionally no significant differences in bead diameter between microcarriers crosslinked at 60°C compared to 37°C demonstrating the high crosslinking temperature did not affect the bead swelling behaviour. MSCs cultured on these microcarriers had a higher cell attachment and twice the proliferation rate compared to the commercial microcarrier Cytodex 1. Unlike in Cytodex 1, cells easily detached under trypsin treatment and did not require extended incubation periods or intense agitation. Furthermore, the possibility of freeze drying the microcarriers was also investigated to reduce storage and transportation costs of the microcarriers.
Supervisor: Ye, Cathy Sponsor: China Regenerative Medicine International Limited
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available