Use this URL to cite or link to this record in EThOS:
Title: Investigating the biophysical properties of ageing of collagenous tissue at the nanoscale
Author: Ahmed, Tarek
ISNI:       0000 0004 7660 4222
Awarding Body: UCL (University College London)
Current Institution: University College London (University of London)
Date of Award: 2019
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Collagen is the most abundant protein in the human body and there are a number of changes that occur to collagen as we age, the main being the accumulation of adventitious advanced glycation end products (AGEs). These can be in the form of covalent cross-links forming between residues within neighbouring collagen molecules. Glucosepane is the most common AGE cross-link found in collagenous tissue and like other AGEs, its impact on the collagen matrix at the nanoscale is not fully understood. This thesis investigates the biophysical properties of collagenous tissue as a function of ageing due to AGE accumulation, in particular Glucosepane. The study identifies nanoscale markers of ageing (morphological and mechanical) and assesses the presence of these markers in various human tissues. The study takes an in-vitro approach to develop glycated tissue models, mimicking the ageing of tissue in the lab. Ex-vivo collagenous tissue samples from donors spanning a variety of ages are also assessed to identify the presence of the markers discovered. The study identifies unique features of the properties of collagen at the nanoscale spurred by age and accumulation of AGEs, including changes in collagen fibrillar structure as well as fibrillar mechanical properties. This thesis proposes a novel collagen-water interaction mechanism which has significant effects on the biophysical properties of collagen as AGEs accumulate.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available