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Title: Investigatdion of bone fracture repair in the presence of perturbed lymphocyte function
Author: Phillips, Sally-Anne
ISNI:       0000 0004 7658 8605
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2014
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Recently the dynamic association between the immune system and the skeletal system has become appreciated. However, to date there has been no attempt at manipulating CD4+ and CD8+ T lymphocytes during fracture repair. This study was aimed at evaluating whether patients and animals with a perturbed lymphocyte function have an abnormal bone fracture repair. The in vivo study involved the creation of a standard closed tibia fracture in BALB -c mice depleted in CD4+ or CD8+ T lymphocytes compared to wild type controls. Bone fracture repair was investigated using radiographs, biomechanical testing, stereology and immunohistochemistry. The CD4+ depleted group had significantly greater callus size at days 7 and 14 compared to controls (p < 0.001). Stereology confirmed a significantly greater proportion of bone than cartilage compared to the CD8+ depleted group. The CD8+ depleted group had a significantly greater callus size at days 7 and 21 post fracture compared to controls (p < 0.005). However stereology demonstrated that the fracture callus had significantly more cartilage and less bone compare to CD4+ depleted, indicating the callus was less mature. This in vivo study has revealed the positive role of CD8+ T lymphocytes on fracture repair and suggested a potential inhibitory role of CD4+ T lymphocytes. Overall this study has suggested that a perturbed lymphocyte function in animals affects fracture repair.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available