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Title: Silver coating of Ti-scaffolds does not affect bone regeneration in vivo or reduce growth of MRSA 43300 in vitro
Author: Devlin-Mullin, Aine
ISNI:       0000 0004 7655 3656
Awarding Body: Ulster University
Current Institution: Ulster University
Date of Award: 2017
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Peri-operative infections of prosthetic hips leads to implant loosening associated with significant morbidity and mortality, resulting in many extra surgical procedures each year and increasing the financial burden associated with this procedure. Small numbers of organisms (102-103) from pathogenic strains including MRSA result in implant failure due to biofilm formation on their surface making prevention of osteolytic infections a high priority in the field of orthopaedic research. In an attempt to inhibit biofilm formation, porous 3D titanium structures manufactured using SLM were further modified with nanolayer of silver using ALD. On titanium scaffolds, MRSA growth was slow with no further reduction observed on silver-coated scaffolds in both bacterial recovery and biofilm formation. Cell viability and ultrastructural examination assays using MC3T3-E1 mouse pre-osteoblasts and SAOS-2 human osteosarcoma cells confirmed cell attachment, growth and viability is supported on porous titanium scaffolds but this was not the case on silver-coated scaffolds. X-ray computed microtomographic, ultrastructural and histomorphometric analyses established that titanium scaffolds coated with a silver nanolayer implanted into a 2 mm subcritical cortical and cancellous bone defects in rat tibia and femoral head respectively promoted robust vascularization and conspicuous bone ingrowth. In conclusion coating titanium implants with a nanolayer of silver does not affect biofilm formation of this stain of MRSA or support growth and viability of MC3T3-E1 and SAOS-2 cells in vitro, however in vivo the silver nanolayer does not disturb normal osseointegration or bone apposition to scaffolds in cortical and cancellous bone. Vascular casting of cortical bone implanted with silver-coated titanium scaffolds paired with SEM revealed robust bone regeneration in addition to evidence of angiogenesis. The results from these studies indicate that coating these scaffolds with a silver nanolayer show promising results in vivo and therefore further exploration for the use of silver coatings in a clinical setting are warranted.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available