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Title: Osteogenic and dermal integration of silver coated titanium scaffolds used to prevent infection of direct skeletal attachment devices
Author: Todd, Naomi
ISNI:       0000 0004 7653 0649
Awarding Body: Ulster University
Current Institution: Ulster University
Date of Award: 2017
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Background: Staphylococcus epidermidis is one of the most common pathogens which results in 3-5% of peri-operative infections. S. epidermidis exhibits strong biofilm properties in surgical sites and direct skeletal attachments. Low numbers of this micro-organism can result in hospitalisation and revision surgeries resulting in extra surgical procedures, increasing morbidity and mortality rates of patients. The aim of this project was to investigate the biocompatibility, cytocompatibiliy and biofilm formation inhibition using solid and porous titanium implants. These implants were manufactured by selective laser melting (SLM) and coaled' with a nano-layer of silver using atomic layer deposition. Methods: HaCaTs, Hs27, HDMEC and Saos-2 cells were cultured for 6, 24 and 48hrs and 103 of S. epidermidis was cultured over 96hrs in the presence of solid TiAg or Ti (control). Cell and bacterial morphology was evaluated by SEM while cell viability was measured via MTT. Viable bacteria were counted using bacterial recovery assays while biofilm formation was assessed using crystal violet staining. Wistar rats received surgical anaesthesia of ketamine (lOOmg/kg) and xylazine (lOmg/kg). Defects were created in the tibia and implanted with solid and porous TiAg and Ti implants. Angiogenic and osteogenic potential was evaluated using vascular corrosion casting imaged by SEM and histological analysis. Results: In vitro studies demonstrated that 5. epidermidis exhibited a 2-log fold reduction in growth. Biofilm formation was also significantly reduced when grown on TiAg. SEM analysis of cell lines revealed they proliferate on the both implant surfaces with no signs of cytotoxicity. MTT evaluation determined no significant difference in viability between solid TiAg and Ti implants. Vascular casts and histological analysis of rat tibiae analysed by SEM showed full infiltration of micro vessels and primitive bone formation after 12 weeks. Conclusion: Our findings demonstrate silver coating on SLM manufactured titanium implants leads to a significant reduction in S. epidermidis biofilm formation while maintaining osseo-integrative and angiogenic properties.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available