Use this URL to cite or link to this record in EThOS:
Title: In vivo and in vitro behaviour of bioactive glass scaffolds
Author: Shi, Xiaomeng
ISNI:       0000 0004 7659 0369
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2019
Availability of Full Text:
Access from EThOS:
Access from Institution:
Bioactive glass scaffolds have the ability to support the regeneration of bone defects as they form a rapid bond with bone and their dissolution products can stimulate new bone formation. A previous in vitro study of ICIE16 foam bioactive glass scaffolds (49.46% SiO2, 36.60% CaO, 6.60% Na2O, 1.07% P2O5, and 6.60 K2O in mol%) by Nommeots-Nomm et al. showed the rapid formation of a calcium-phosphate rich layer thatmineralised into hydroxycarbonate apatite (HCA), which has similar composition to the mineral phase of bone. Although foam scaffold pore networks mimic the architecture of cancellous bone, control of pore size and strut size is limited. Nommeots-Nomm et al. also employed the robocasting technique which enabled precisely control of pore morphology and strut size. In this study, the printed ICIE16 scaffolds were used as templates for in vitro synthesis of bone tissue using human bone marrow derived stem cells (hBMSC). The printed ICIE16 scaffolds stimulated osteogenic differentiation of the hBMSCs under both basal and osteogenic conditions. Two types of ICIE16 bioactive scaffolds (foam versus printed) with similar interconnected pore sizes were evaluated using a rabbit femoral head defect model to investigate the effect of pore morphology. The printed group showed similar bone ingrowth 23.64 ± 3.62% than the foam and control group and the printed scaffolds were not fully degraded after 10 weeks. A new composition of glass (49.5% SiO2, 30.5% Ca2O, 4.0% Na2O, 4.5% P2O5, 4.0% K2O and 7.5 MgO in mol.%) together with a control group with half of the calcium substituted by strontium was synthesised and fabricated into 3D scaffolds using robocasting techniques. In vitro analysis was completed to understand the effect of strontium on dissolution behaviour and HCA formation. Incorporation of strontium in a glass system was shown to reduce the increase in pH of the SBF solution and inhibit the dissolution rate of the modifier ions.
Supervisor: Jones, Julian Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral