Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.768430
Title: Alternative electron transfer pathways in iron-metabolising bacteria
Author: Gomez Perez, Laura
ISNI:       0000 0004 7654 1364
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2018
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Abstract:
Ferric iron (Fe3+) can be used as a terminal electron acceptor by iron-reducing microorganisms to facilitate cellular respiration under anoxic conditions. In contrast, ferrous iron (Fe2+) is used as an electron donor by iron-oxidising microorganisms. The presence of dissimilatory pathways in iron metabolisers maintain bioavailable iron in the environment for other organisms. Redox reactions between gram-negative bacteria and iron usually occur through an extracellular electron transport (EET) pathway that allows electrons to cross from the inner membrane to the extracellular environment or vice versa. The study of the redox pathway has been thoroughly studied in the iron reducer Shewanella oneidensis, however, there is little information about the metabolic pathways used by other iron metabolisers. In this thesis, the environmental isolates Acinetobacter and Citrobacter have been described as novel iron reducing bacteria by using a combination of techniques including ferrozine assays, cytochrome identification methods and transcriptomic analysis. Results in this thesis suggest that Acinetobacter (previously described as a strict aerobic microorganism) in fact is capable of respiring using iron when oxygen is not available. In contrast, Citrobacter's iron reduction pathway seems to involve fermentative processes. These results point out that extracellular electron transport is not the only mechanism in dissimilatory iron metabolism. Moreover, these results suggest that anaerobic environments could be a reservoir for pathogenic strains of Acinetobacter, as it has been shown that this species do not require oxygen to survive. In addition to the study of new iron reducers, an overexpression system has been developed to study proteins involved in metal oxidation pathways without the need of culturing the notoriously slow-growing iron oxidising bacteria. The genes cyc2 and cyc2PV-1, which encode outer membranes cytochromes from Acidithiobacillus ferrooxidans and Mariprofundus ferrooxydans respectively, have been transformed into the iron reducer Shewanella oneidensis for protein expression optimization. The optimization of this process offers great possibility for the future study and applications of metal oxidation pathways.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.768430  DOI: Not available
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