Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.766436
Title: Design and synthesis of novel cytochrome bc1 inhibitors for the treatment of toxoplasmosis
Author: Gordon, James Alexander
ISNI:       0000 0004 7654 8742
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2018
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Abstract:
Toxoplasmosis is a highly prolific and neglected disease, and current treatments fail to adequately address the challenges it presents. As such development of new more efficacious treatments, tailored to the complex nature of the infection, is required. The causative parasite Toxoplasma gondii is a resilient and resourceful organism, adept at invasion, avoidance and survival within its host. The cytochrome bc1 complex has been proven to be a validated target for the treatment of a number of apicomplexan parasitic diseases, including both malaria and toxoplasmosis. Recent work has also identified overexpression of this complex within a strain expressing the difficult to treat bradyzoite phenotype, which supported by findings that treatment with known bc1 inhibitor atovaquone shows reduction in cyst burden, suggests this may be a particularly promising target for an effective systemic parasite treatment. Within discusses the design, synthesis and analysis of a number of cytochrome bc1 inhibitors across several novel scaffolds. Designs focused on improving deficiencies identified in previous inhibitors. Successful synthesis of a number of these designs and biological evaluation identified several submicromolar examples. After preliminary ADMET evaluation of the synthesised scaffolds the tetrahydroquinolone scaffold was selected for further development. Optimisation of the synthesis to the tetrahydroquinolones (THQ's) resulted in access to a library of analogues. From these analogues compound 169 was selected for further analysis, characterisation and ultimately progressed to early stage in vivo assays, in which it demonstrated exceptional potency and efficacy.
Supervisor: Fishwick, Colin W. G. Sponsor: University of Leeds
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.766436  DOI: Not available
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