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Title: The role of adipose tissue in canine cranial cruciate ligament disease
Author: Saengsoi, Wipawee
ISNI:       0000 0004 7658 7397
Awarding Body: University of Liverpool
Current Institution: University of Liverpool
Date of Award: 2018
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Cranial cruciate ligament disease (CCLD) is one of the most important causes of pelvic limb lameness in dogs, and its prevalence is increased in dogs that are overweight. To date, the mechanisms underlying the association between obesity and CCLD in dogs have not been clarified. Therefore, the general aims of this thesis were to examine the role of both the mechanical and humoral effects of adipose tissue on the clinical presentation and pathogenesis of dogs with CCLD. Dogs suffering from CCLD were clinically evaluated by calculating lameness scores, force plate analysis, radiographic examination, and dual-energy X-ray absorptiometry (DEXA). Increased bodyweight was associated with severity of lameness, as determined by force plate analysis, but there was no association with body fat mass determined by DEXA. In vitro studies were also used to examine interactions between adipose tissue and the canine cruciate ligament. In co-culture experiments, adipose tissue had a catabolic effect on glycosaminoglycan content in CCL explants. Furthermore, various associations between key adipokines (adiponectin, leptin, visfatin) with disease biomarkers in CCL tissues and joint fluid were discovered. For example, an association was identified between synovial fluid TNF-a concentration and the gene expression of adiponectin and visfatin in infrapatellar fat pad (IPFP) adiponectin and subcutaneous (SC) fat, respectively. Associations were also seen between IPFP leptin gene expression and MMP-13 gene expression, and between both IPFP and SC visfatin gene expression and CCL TNF-a gene expression. Finally, synovial fluid leptin concentration was positively associated with the degree of lameness. In conclusion, the studies undertaken in this thesis have demonstrated that adipose tissue may have both mechanical and humoral effects on the cruciate ligament in dogs. These findings provide a basis for further studies into the pathogenesis of CCLD, as well as revealing possible targets for therapeutic intervention.
Supervisor: German, Alex ; Comerford, Eithne ; Tew, Simon Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral